The location of the Nest room is unknown. If you must deploy enemies to the Nest while it is inactive, deploy enemies face-down to the Nest Stack instead. Do not look at the front of an enemy card before deploying it to the Nest Stack.
When the Nest is revealed, the enemies in the Nest Stack deploy to the Nest. If the Nest has been removed from play, enemies cannot be deployed to the Nest Stack.
If the Self-Destruct Sequence activates while there are enemies in the Nest Stack, they immediately deploy to the Cryo Chamber, even if it is inactive.